Abstract Paper aims This study has the objective of mapping and empirically validating KPIs that can be used to assess healthcare supply chain risks. Originality We combined two methodological approaches: i) we conducted a systematic literature review to identify the healthcare supply chain risk assessment KPIs and ii) We grouped these KPIs into five constructs and validated their relations using PLS-SEM. Research method This is the only study that presents a systematic literature review to identify KPIs that measure healthcare supply chain risks, groups the KPIs into a theoretical framework, and presents a PLS-SEM validation. This study used data from Brazil, from a variety of healthcare supply chain organizations (suppliers, OEMs, clinics, and hospitals), therefore we cannot guarantee that the study can be generalized. Main findings The systematic literature review identified 27 KPIs which were grouped into five constructs. The structural model supported by the PLS-SEM technique revealed that reliability, responsiveness, costs, and agility are antecedents to healthcare supply chain quality. Implications for theory and practice Managers may rely upon this study to understand that healthcare supply chain reliability and responsiveness are antecedents to healthcare supply chain costs and agility, which are antecedents to healthcare supply chain quality.